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Alkyne-Activated Fluorogenic Azide Probes

Details

Project TitleAlkyne-Activated Fluorogenic Azide Probes
Track Code24000
Websitehttps://techtransfer.universityofcalifornia.edu/NCD/24000.html?utm_source=AUTMGTP&utm_medium=webpage&utm_term=ncdid_24000&utm_campaign=TechWebsites
Short DescriptionNone
Abstract

Significant progress has been made in the discovery of fluorogenic probes activated by various bioorthogonal chemistries, including the Staudinger ligation as well as azide-alkyne, tetrazine-alkene, tetrazine-alkyne, and photoactivated tetrazole-alkene cycloadditions. However, the dyes employed have emission maxima below 600 nm. The identification of activatable near infrared (NIR) fluorogenic probes with emission maxima greater than 600 nm has proven much more challenging. There is a clear need in the art for methods of labeling biomolecules in vitro or in vivo.

Scientists at UC Berkeley have developed NIR fluorogenic azide probes and the methods for labeling a target biomolecule using these compounds based on Si-rhodamine scaffold that undergo a fluorescence enhancement of up to 48-fold upon reaction with terminal or strained alkynes. These xanthene fluorogenic azide probes consist of xanthene fluorophores containing functionalized pendant aryl rings. This azide switch quenches fluorescence via photo induced electronic transfer. As the probes leverage the inherent emission properties of the xanthane fluorophore, these fluorogenic azide probes fluoresce from the green through the NIR at commonly used excitation and emission wavelengths. They used the probes for mammalian cell surface imaging and, in conjunction with a new class of cyclooctyne D-amino acids, for visualization of bacterial peptidoglycan without the need to wash away unreacted probe.

 
TagsGenomics, proteomics, Diagnostics, imaging, Research Tools, screening
 
Posted DateJul 12, 2017 7:50 AM

Advantages

  • No need to wash away unreacted probe.
  • Can help develop probes for in vivo studies.
  • No need of using cytotoxic copper catalyst.

Potential Applications

  • Detection of biomolecules.
  • Detection of alkynes in vitro.
  • Detection of binding interactions via proximity-accelerated click reactions.
  • Ideal for imaging studies.

 

Additional Information

Related Materials




Additional Technologies by these Inventors



Tech ID/UC Case

24000/2014-147-0



Related Cases

2014-147-0

Contact Information

Name : Javed Afzal

Title :

Department :

Email : jafzal@berkeley.edu

Phone : 510-643-7201

Address :

Principal Investigator

Name : Carolyn Bertozzi

Department :



Name : Peyton Shieh

Department :

Intellectual Property

Patent Number : 9410958

Patent Title :

Patent Application Date :

Patent Publication Date :

Patent Issue Date : Aug 9, 2016

Patent Link : http://www.google.com/patents/US9410958