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Intense, long-lasting fluorescent microtubule-binding contruct (Protein Conjugates Containing Multimers of Green Fluorescent Protein)

Information

Organization Name Columbia Tech Ventures
Institutional ID Number 7004
Technology Tags or Keywords
Summary

This construct expresses the microtubule-binding domain of ensconsin in conjunction with three copies of the green fluorescent protein gene.

Technology Benefit

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Technology Applications

The construct is excellent for visualizing microtubules as fluorescence is intense and long-lasting, without changing the behavior of the cell. It can also be used to develop cell lines stably expressing fluorescently-labeled microtubules.

Technology page URL http://techventures.columbia.edu
Detailed Technology Description

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TTO homepage URL --
URL to link to documents, images, videos, etc. --
Additional Information
Track Code
P7004
Publications
E-MAP-115 (ensconsin) associates dynamically with microtubules in vivo and is not a physiological modulator of microtubule dynamics
Principal Investigator
Bulinski, J. Chloe
Construct Information

Type of vector: Bacterial/mammalian

Insert name: Ensconsin microtubule-binding domain

Fusion tag: 3xGFP

Name of vector used: pEGFP-N1

Inquiry

Jullian G. Jones, Ph.D., J.D.

80 Claremont Avenue, 4th Floor, MC 9606

New York, NY 10027

Tel: 212-851-0258

Email: TechTransfer@columbia.edu