Organization: University of Michigan
Hybridoma Cell Line 1E7E8, Monoclonal Antibody UMCD2
Organization: University of Michigan
Treatment with an Antibody Against VGamma4 T Cells Reduces Rheumatoid Arthritis Symptoms in Mice
Organization: National Jewish Health
Our scientists have shown in a collagen-induced arthritis murine model: Increased numbers of activated Vgamma4 cells in active disease Vgamma4 cells were major producers of the inflammatory cytokine, IL-17 Administration of a monoclonal antibody targeting Vgamma4 T cells in mice resulted in a 42% decrease in RA histological parameters and a significant decrease in autoantibodies
Organization: University of Maryland
EGFR Inhibitors as Therapeutic Agents for Breast Cancer Bone Metastasis
Organization: Princeton University
Princeton University Invention # 09-2509 The involvement of the EGFR signaling pathway in bone metastasis has been implicated by a number of studies. However, the therapeutic significance by targeting EGFR signaling in the tumor-stroma interface has not been evaluated. Researchers in the Molecular Biology Department at Princeton University have used a preclinical model of breast cancer bone metastasis to ...
Treating Carcinomas Using Immunotoxin Specific for Cancer Stem Cells
Organization: University of Minnesota
IMMUNOTOXIN FOR CANCER STEM CELLS TARGETING CANCER STEM CELLS TO PREVENT TUMOR RECURRENCE Carcinomas are invasive malignant tumors of transformed epithelial cells. It has been proposed that tumor cells derive from a small population of cancer stem cells (CSCs) – self-generating progenitor cells that can migrate, replicate, and differentiate into mature cancer cells. CSCs appear resistant to chemotherapy drugs and radiation treatment. Although the majority of cancer cells may be destroyed with traditional treatment, tumor ...
West Nile Virus Diagnostic Microsphere Immunoassay
Organization: Health Research Inc
The current recommended assays for the identification of West Nile Virus infection of humans are the IgM antibody capture enzyme linked immunosorbent assay (ELISA) and the IgG ELISA. Many laboratories in the US are performing these assays according to protocols recommended by the Centers of Disease Control and Prevention (CDC). This combination of assays is highly sensitive and specific, but requires several days to weeks, and specialized facilities to perform the complete panel of tests. Our nonstructural protein assays can be completed in ...
Method of Treating Multiple Myeloma by Targeting CD28
Organization: University of Miami
The current invention relates to novel methods that use therapeutic monoclonal antibodies to CD28 found on myeloma cells. Monoclonal antibodies against CD28 may: 1.) be directly lethal to myeloma cells (via antibody-mediated complement targeting), 2.) prevent myeloma proliferation (by blocking CD28 function on myeloma cells), and/or 3.) be toxic to myeloma cells after conjugation to radioisotopes or cellular toxins.
GFAbs – GFP-Based Biosensors with the Binding Properties of Antibodies
Organization: Wisconsin Alumni Research Foundation (WARF)
Antibodies are used widely in biological and medical research to bind to and help isolate molecules of interest. However, the reduced stability and production yields of antibody fragments frequently utilized in in vitro platforms has led to attempts to create alternative binding scaffolds. By mutagenizing solvent-exposed loop regions or inserting diverse loop repertoires into non-antibody protein scaffolds, specific binding attributes can be conferred to proteins with desirable properties, such as high stability and production ...
KKO Monoclonal Anti-PF4/Heparin antibody
Organization: STC UNM
The invention is a murine monoclonal antibody to PF4 and heparin that behaves nearly identically to the human antibodies that cause disease. Our monoclonal anti-PF4/heparin antibody, designated KKO™, has several features that are critically similar to the human antibodies, including: heparin-dependent reactivity in solution and to endothelial cell bound PF4, binding to complexes of PF4 with other carbohydrate moieties, and most importantly, platelet activation in the presence of PF4 and heparin. KKO™ is the first monoclonal ...
Organization: Hospital for Sick Children
The present invention provides for the identification of DIO individuals at risk of developing MetSyn and/or T2D, and the subsequent regulation of DIO and/or obesity associated metabolic diseases such as insulin resistance, hyperinsulinemia, glucose intolerance, liver steatosis, dyslipidemia and progression to MetSyn and T2D. Visceral adipose tissue (VAT) from DIO mice and humans progressively accumulates gamma-IFN secreting Th1 cells that overwhelm static numbers of T-cells. Therapeutic enhancement of T-cells in VAT following brief systemic ...
Peptide Vaccine for Chronic Lymphocytic Leukemia
Organization: University of California, San Diego (UC San Diego)
ROR1 is a tyrosine-protein kinase transmembrane receptor whose expression has been associated with cancer and metastasis. UCSD researchers designed ROR1 peptides and, when conjugated with an immunogenic carrier, and were able to elicit an immune response.
Monoclonal Antibodies to Identify and Remove Teratoma-initiating Stem Cells
Organization: Stanford University
Monoclonal Antibody MB-1 (CD37)
Organization: Stanford University
Human-specific anti-SMN antibody (clone 4F11)
Organization: University of Missouri Columbia
STATE OF DEVELOPMENT: Ready to use. LICENSING POTENTIAL: University seeks licensee with potential commercialize.
Clinical Immunoassays to Determine Concentration of Monoclonal Antibodies
Organization: Indiana Univ-Purdue University Indianapolis
This technology is a general method to develop assays to detect the circulating concentration of monoclonal antibodies in patients that have received them as treatment for cancer or other diseases. The method is applicable to any monoclonal antibody or other recombinant protein biologic therapy. In addition, two specific assays, one for rituximab and one for alemtuzumab, have been developed. Synthetic biotinylated peptides have been produced based on the sequence derived from phage displayed peptide libraries. UCSD researchers have then ...
Anti-Insulin Degrading Enzyme Antibody
Organization: Indiana Univ-Purdue University Indianapolis
Monoclonal Antibodies Against a Cancer Marker
Organization: Indiana Univ-Purdue University Indianapolis
UM File # 2164BackgroundExisting cancer screening tests are often invasive, expensive, and lack strong diagnostic utility. For example, prostate cancer is typically diagnosed with a digital rectal exam and/or prostate specific antigen (PSA) screening, which has limited sensitivity and specificity. Thus, development of additional serum and tissue cancer biomarkers is needed to supplement current screening methods.TTechnology DescriptionResearchers at the University of Michigan have identified HIP1 (Huntingtin Interacting Protein 1) as a novel ...
Organization: Indiana Univ-Purdue University Indianapolis
UM File # 2376BackgroundVimentin is a widely expressed intermediate filament that can be secreted by activated human macrophages thus playing a role in immune response and elimination of bacteria. Exploration of this protein's activity may lead to treatment in either inflammatory diseases or antibacterials. While there are multiple medications for inflammatory diseases, these medications have serious limitations in both therapeutic efficacy and side effect profiles. Therefore, while one can indeed treat these disease entities, there is ...
Monoclonal Antibody Specific for the von Willebrand Factor
Organization: Indiana Univ-Purdue University Indianapolis
Organization: Indiana Univ-Purdue University Indianapolis
UM File # 2555BackgroundIn the United States, colorectal cancer is the second leading cause of all cancer deaths. If the cancer is found and treated early before it spreads to lymph nodes or other organs, the survival rate is higher. However, less than 40% of colon cancers are discovered at an early stage. Colon cancer is diagnosed by fecal occult blood test, digital rectal examination, barium enema, or sigmoidoscopy or colonoscopy. The current available diagnostic techniques are limited in their ability to decisively identify and ...
Identification of Phosphorylated Tyrosines Within the Proteins SH2-B and the Protein JAK2
Organization: Indiana Univ-Purdue University Indianapolis
UM File # 2544BackgroundTechnology DescriptionApplicationsAdvantages
Monoclonal and Polyclonal Antibodies
Organization: Indiana Univ-Purdue University Indianapolis
UM File # 2809BackgroundTechnology DescriptionApplicationsAdvantagesveloped a series of monoclonal antibodies and polyclonal antibodies against NRL (a bZIP transcription factor expressed in rod photoreceptors and pineal gland) and NR2E3 (a photoreceptor specific nuclear receptor)
Organization: Indiana Univ-Purdue University Indianapolis
UM File # 2835BackgroundTranscription factor p53 has been known as a tumor suppressor. It can induce growth arrest and/or apoptosis in cells through activation or repression of downstream target genes. Zinc-finger binding protein-89 (ZBP-89) cooperates with histone acetyltransferas coactivator p300 in the regulation of p21, a cyclin-dependent kinase inhibitor, whose associated gene is a target gene of p53. Recent research suggests that ZBP-89 is able to induce growth arrest and apoptosis in human gastrointestinal cell lines through ...
Process for Preparing Single Enantiomers of Fluoroleucine Compounds
Organization: Indiana Univ-Purdue University Indianapolis
UM File # 2931BackgroundFluorocarbons have long been known for their chemical inertness, and their unique properties have found industrial and medical uses as fire retardants, refrigerants, anesthetics and biologically inert polymers. Recently, there has been much interest in whether fluorocarbon polymers can be exploited in the design of biological macromolecules, given their strong self-association and an affinity for lipid bilayers. Extensively fluorinated analogs of hydrophobic amino acids, when substituted into proteins and peptides, ...
Antigen for Pax2 Monoclonal Antibody Generation
Organization: Indiana Univ-Purdue University Indianapolis
UM File # 3764BackgroundPax-2 belongs to a family of homebox genes that contain a paired domain, which binds to the DNA to initiate transcription of specific genes. As the first known kidney-specific gene to be expressed in the pronephros of the mouse embryo, Pax-2 is indispensable for ureteric bud development. Recent, scientists have found that activation of Pax-2 can contribute to a variety of renal diseases. Thus, Pax-2 can be a potential therapeutic target for renal diseases.Technology DescriptionThe C-terminal of Pax-2 is the ...
Antibody for Acute and Chronic Inflammatory Diseases
Organization: Indiana Univ-Purdue University Indianapolis
UM File # 3810BackgroundToll like receptors (TLRs) are a family of conserved pathogen recognition receptors that are integral to innate immune responses. Among the 13 TLR members in mammal, TLR3 recognizes viral PAMPs. The role of TLR3 in viral-induced pathology has been extensively studied in the context of gene deficient mice. TLR3 activation following viral infection appears to elicit two major responses: effective immunity and/or bystander tissue injury. The latter aspect of TLR3 activation has been shown as TLR3 deficient mice show ...
Inhibitors of Metastasis and Angiogenesis
Organization: Indiana Univ-Purdue University Indianapolis
UM File # 4972BackgroundCancer is a major public health problem in the United States and many other parts of the world. Currently, one in 4 deaths in the United States is due to cancer, and cancer care accounts for over $105 billion in healthcare spending each year. Drugs for the treatment of cancer can be broadly classified into two categories: traditional cytotoxic chemotherapies and molecularly targeted drugs. Targeted drugs are developed to specifically alter the activity of one or more proteins involved in cancer. Targeted monoclonal ...
Organization: Indiana Univ-Purdue University Indianapolis
This novel technology is a way to diagnose and treat CLL, breast cancer, and other cancers. A monoclonal antibody has been generated by UC San Diego against a receptor which is implicated in the progression of cancer. This protein named ROR1 is expressed on CLL B cells, but it is not present in normal lymphocytes.
Monoclonal Mouse lgG and lgM Antibodies that Neutralize Yellow Fever Virus
Organization: Indiana Univ-Purdue University Indianapolis
This invention presents serious of monoclonal mouse IgG and IgM antibodies against yellow fever virus and dengue fever, and the antibody-producing hybridoma cell lines. These antibodies have multiple applications in production of vaccine against yellow fever. These antibodies are These antibodies have been proved to be very helpful in testing, quality control, and manufacturing vaccines against yellow fever and dengue fever. Antibodies available are for NS1 and E protein of dengue and yellow fever virus.
Passive Intranasal Monoclonal Antibody Prophylaxis Against Murine Pneumocystis Carinii Pneumonia
Organization: Indiana Univ-Purdue University Indianapolis
This research has identified a conserved immunogenic epitope on P. carinii and Streptococcus pneumoniae that is dually recognized by a monoclonal antibody, 4F11. The peptide containing the protective epitope can be utilized to develop a vaccine to prevent or treat disease caused by P. carnii or S. pneumoniae. Recent work has demonstrated that immunization with a recombinant protein containing the antigen produces an antibody response that recognizes P. carinii antigens in a mouse model. Additionally, the 4F11 antibody recognizing the epitope ...
Non-Invasive, Sensitive Diagnostic for Viral Myocarditis
Organization: Indiana Univ-Purdue University Indianapolis
Researchers from UC San Diego have identified altered forms of a protein complex present in cardiac muscle (the dystrophin-glyoprotein complex), which is associated with dilated cardiomyopathy caused by enteroviruses. Specifically, the presence of a unique, enteroviral protease 2A-cleavage product provides a highly specific and sensitive biomarker for enteroviral-mediated cleavage of dystrophin.
Organization: Indiana Univ-Purdue University Indianapolis
Human androgen receptor (residues 331-572) Monoclonal Antibody
Organization: Indiana Univ-Purdue University Indianapolis
Human progesterone receptor Monoclonal Antibody
Organization: Indiana Univ-Purdue University Indianapolis
Murine Ly-6C (T cell activating)
Organization: Indiana Univ-Purdue University Indianapolis
Organization: Indiana Univ-Purdue University Indianapolis
Full length enhanced green fluorescent protein (eGFP) Monoclonal Antibody
Organization: Indiana Univ-Purdue University Indianapolis
Monoclonal Antibody Directed against Human Type V Phospholipase A2 (hVPLA2)
Organization: Indiana Univ-Purdue University Indianapolis
Mouse H2-M3 MHC Class Ib Monoclonal Antibody
Organization: Indiana Univ-Purdue University Indianapolis
Methods of Therapy for Non-Hodgkin's Lymphoma
Organization: Indiana Univ-Purdue University Indianapolis
The present invention offers a method for treating non-Hodgkin's B-cell lymphoma using a combination of interleukin-2 (IL- 2) or variant, and at least one anti-CD20 antibody or fragment. Administration of IL-2 expands and activates natural killer (NK) cells, leading to enhanced antibody-dependent cellular cytotoxicity of anti-CD20 antibody. This combination treatment significantly improves the effectiveness of the current rituximab (anti-CD20 monoclonal antibody) therapy.
Modified Avidin -Biotin Technique
Organization: Indiana Univ-Purdue University Indianapolis
In indirect enzyme-linked immunohistochemical procedures, the secondary antibody serves the pivotal role of linking the primary antibody bound to the antigen of interest in a tissue section with a tertiary complex containing an enzyme reporter molecule. One of the most common methods for this is the avidin-biotin-complex (ABC) method. This method is simple, has high signal amplification, and low background noise. These features of the ABC method have prompted its widespread use as an immunohistochemical detection system. There is, ...
Methods for Production and Use of Saccular Collagen
Organization: Indiana Univ-Purdue University Indianapolis
Collagen is the main constituent of skin, connective tissue, and bone. Several types of collagen have been identified which differ slightly in their primary structure and biochemical properties. University of Pennsylvania researchers have isolated a new member of the collagen protein family, termed saccular collagen, which contains the smallest known triple helix domain. Antibodies to saccular collagen have also been isolated to aid in the purification of saccular collagen from natural sources.
Organization: Indiana Univ-Purdue University Indianapolis
AlzheimerÃs disease is characterized by senile plaques, neurofibrillary tangles (NFTs) and related neurofibrillary lesions. To further characterize these abnormalities, researchers at the University of Pennsylvania raised monoclonal antibodies against extracts of these lesions, which were used as immunogens. Of the antibodies generated, four were further characterized because of their unexpected immunoreactivity, with regard to NFTs or the hyperphosphorylated tau protein which comprises NFTs. However, these antibodies did react with a ...
Method of Diagnosing Alzheimer's disease
Organization: Indiana Univ-Purdue University Indianapolis
The main pathologic signs of AlzheimerÃs disease are senile plaques and neurofibrillary tangles, which represent alterations in the cytoskeletal apparatus. The principle structural elements of neurofibrillary tangles are paired helical filaments (PHFs) w
Method of Stimulating Nerve Myelination
Organization: Indiana Univ-Purdue University Indianapolis
Myelin destruction of nerve cells is the main pathology of demyelinating neuropathies, such as multiple sclerosis. The binding of a viral receptor expressed on oligodendrocytes stimulates the appearance of components of myelin biosynthesis, laying the gr
Methods of Treating Cancerous Cells with Anti-Receptors Antibodies
Organization: Indiana Univ-Purdue University Indianapolis
Currently, tumor treatments are primarily based on the cytotoxic effects of drugs and radiological therapy. Despite the fact that these treatments offer some remission, they unfortunately have side effects, killing not only tumor cells but also normal non
Prevention of Tumors with Monoclonal Antibodies Against the Neu Gene Product
Organization: Indiana Univ-Purdue University Indianapolis
University of Pennsylvania researchers have developed a method to prevent mammalian tumors which contain cells expressing the neu gene product, p185. p185 is a transmembrane glycoprotein with tyrosine kinase activity. p185 has been found active in human
Methods of Constructing and Using Biologically Active Compounds to Mimic or Block Natural Proteins
Organization: Indiana Univ-Purdue University Indianapolis
Protein-protein interactions are important in many biological functions, including the immunological response to pathogenic antigens. These interactions include a protein or pathogen binding to a receptor, protein interactions at cellular attachment site
Organization: Indiana Univ-Purdue University Indianapolis
Researchers have developed a new method of producing vaccines to any infectious organism that binds specifically to mammalian host cells, but in particular to viruses, including reoviruses and rotaviruses. Monoclonal antibodies are produced against the b
Organization: Indiana Univ-Purdue University Indianapolis
This technology, developed at the University of Pennsylvania, comprises an adaptation of, and improvement to, a commercially available cell panning methodology. In addition to facilitating red blood cell antigen typing, this invention adapts the conventi
Monoclonal antiody reactive with HIV co-factor CXCR4
Organization: Indiana Univ-Purdue University Indianapolis
University of Pennsylvania researchers have developed an antibody for the HIV co-factor Fusin. Fusin was recently identified and shown to be required as a co-factor with CD4 to form a competent viral receptor. Fusin is a member of a family of molecules t
An Attenuated Strain of Listeria Monocytogenes for Use as a Vaccine Vector
Organization: Indiana Univ-Purdue University Indianapolis
Because cytotoxic T cells (CTLs) can potentially destroy HIV infected cells and might prevent the dissemination of the infection at early times in an infected individual, generation of a strong CTL response may be useful as a prophylactic or therapeutic v
Targeting of Drugs to the Luminal Surface of the Pulmonary Endothelial Cells
Organization: Indiana Univ-Purdue University Indianapolis
Fibrinolytic therapy with plasminogen activators (PA) is limited by its extremely rapid elimination from the bloodstream due to the uptake by a non-target tissues. Researchers at the University of Pennsylvania have developed a novel technology that allows
Organization: Indiana Univ-Purdue University Indianapolis
Traditional methods for protein detection and quantification, such as 2-D gel electrophoresis, mass spectrometry, and antibody binding, all suffer from relative insensitivity. Newer technologies that have improved the ability to monitor various molecules
Shared Specificity Monoclonal Antibodies Against Both p185 her2/neu and EGFr
Organization: Indiana Univ-Purdue University Indianapolis
Significant time and money have been spent searching for mammalian cancer tumor treatments. Current tumor treatments rely on the cytotoxic effects of drugs and radiological therapy. Although these treatments bring remission and cure to some patients, they
Compounds that are Immunologically Crossreactive with Antibodies
Organization: Indiana Univ-Purdue University Indianapolis
Many pathogens attack host cells through receptor-mediated interactions. Previous attempts to synthesize compounds (i.e. antibodies) against such receptors in an effort to block pathogen binding have met with difficulty because such compounds were, thems
Panning Phage Display Libraries Using Magnetically-Activated Cell Sorting
Organization: Indiana Univ-Purdue University Indianapolis
The ability to produce monoclonal antibodies has revolutionized diagnostic and therapeutic medicine. However, for many human applications, it is desirable to produce human antibodies. There remains a need for a method of isolating recombinant proteins, p
Compounds and Methods of Detecting Hypoxia
Organization: Indiana Univ-Purdue University Indianapolis
Hypoxic cells are the most familiar form of treatment-resistant cells found in certain tumors, vascular diseases or after a stroke. These cells have an inadequate supply of blood and thus have very low concentrations of oxygen, which allow resistance to
Neutralizing human monoclonal antibodies to H5N1 influenza viruses
Organization: Indiana Univ-Purdue University Indianapolis
Rabbit Anti-human Merlin Polyclonal Antibody
Organization: Indiana Univ-Purdue University Indianapolis
Zebrafish epidermal antibody array
Organization: Indiana Univ-Purdue University Indianapolis
TMPRSS2 monoclonal antibody (P5H9-A3)
Organization: Indiana Univ-Purdue University Indianapolis
Organization: Indiana Univ-Purdue University Indianapolis
Organization: Indiana Univ-Purdue University Indianapolis
Organization: Indiana Univ-Purdue University Indianapolis
Organization: Indiana Univ-Purdue University Indianapolis
Monoclonal Antibody to Drosophia Rho1 GTPase
Organization: Indiana Univ-Purdue University Indianapolis
Monoclonal Antibody 66.1 [anti CD4 IgM]
Organization: Indiana Univ-Purdue University Indianapolis
Organization: Indiana Univ-Purdue University Indianapolis
Monoclonal Antibodies to Sphere Organelle Protein [Hybridoma H1]
Organization: Indiana Univ-Purdue University Indianapolis
Monoclonal Antibodies to SR Proteins [Hybridomas: 16H3, 1H4, 7B4]
Organization: Indiana Univ-Purdue University Indianapolis
Monoclonal antibodies to MBP (maltose binding protein P2F1)
Organization: Indiana Univ-Purdue University Indianapolis
Monoclonal antibodies to GST (P1A12)
Organization: Indiana Univ-Purdue University Indianapolis
Monoclonal Antibodies to Canine CD34
Organization: Indiana Univ-Purdue University Indianapolis
Monoclonal antibodies (mAb) specific for the NKG2D ligands ULBP-1, -2, -3, -4, and -5
Organization: Indiana Univ-Purdue University Indianapolis
Monoclonal Antibodies directed to the 6H tag P5A11
Organization: Indiana Univ-Purdue University Indianapolis
Melanoma/Carcinoma Antibodies [96.5]
Organization: Indiana Univ-Purdue University Indianapolis
Mammalian CTCF and Compositions and Methods Relating Thereto
Organization: Indiana Univ-Purdue University Indianapolis
Hybridoma Producing Anti-HLA-G Antibodies
Organization: Indiana Univ-Purdue University Indianapolis
Hybridoma P67.6 [anti-CD33, IgG1]
Organization: Indiana Univ-Purdue University Indianapolis
Organization: Indiana Univ-Purdue University Indianapolis
GP140/CDCP1 as a mediator of binding and internalization of bacteria in epithelium
Organization: Indiana Univ-Purdue University Indianapolis
Galloway Fusion Proteins and Polyclonals
Organization: Indiana Univ-Purdue University Indianapolis
Organization: Indiana Univ-Purdue University Indianapolis
Anti-Ubiquitin Monoclonal Antibodies [Hybridomas: P1A6, P4G7, P4D7, P4D1]
Organization: Indiana Univ-Purdue University Indianapolis
Antibody recognizing the WASH subfamily of WASP (Wiskott-Aldrich syndrome) protein family
Organization: Indiana Univ-Purdue University Indianapolis
Organization: Indiana Univ-Purdue University Indianapolis
Organization: Indiana Univ-Purdue University Indianapolis
Anti-Gab2 (p97/p100) polyclonal antibody
Organization: Indiana Univ-Purdue University Indianapolis
Organization: Indiana Univ-Purdue University Indianapolis
Organization: Indiana Univ-Purdue University Indianapolis
Organization: Indiana Univ-Purdue University Indianapolis
Monoclonal Antibodies against HDAC11
Organization: German Cancer Research Ctr (DKFZ)
Two hybridoma cell lines were created using standard cell culture technique. Mice were im-munized with recombinant HDAC11 protein of whole HDAC11 variant 1 sequence.
Rabbit Anti-human Merlin Polyclonal Antibody
Organization: University of Cincinnati
The neurofibromatosis type 2 (NF2) tumor suppressor gene was cloned in 1993. It encodes a protein product, merlin. Mutation of merlin is associated with development of tumors known as schwannomas, which arise from the 8th cranial nerve. Patients of NF2 are also predisposed to other nervous system tumors such as ependymomas, meningiomas and mesotheliomas. Merlin is related to the ezrin-radixin-moesin (ERM) family of proteins, which are known to link the plasma membrane of cells to their cytoskeleton. Current evidence points to merlin as part ...
Monoclonal Antibodies to Identify and Remove Teratoma-initiating Stem Cells
Organization: Stanford University
Monoclonal Antibody MB-1 (CD37)
Organization: Stanford University
Beryllofluoride Mimics The Acyl Phosphate Linkage In Bacterial 'receiver' Domains
Organization: University of California, Berkeley (UC Berkeley)
Hybridoma 18d3, Producing Monoclonal Antibody Specific For Mouse Cd94
Organization: University of California, Berkeley (UC Berkeley)
Hybridoma 2f1, Producing Monoclonal Antibody Specific For Mouse Klrg1
Organization: University of California, Berkeley (UC Berkeley)
Hybridoma 14b11, Producing Monoclonal Antibody Reactive With Mouse Ly49c, Ly49f, Ly49h And Ly49i
Organization: University of California, Berkeley (UC Berkeley)
Hybridoma (17b5), Producing Monoclonal Antibody Specific For Mouse 4-1bb
Organization: University of California, Berkeley (UC Berkeley)
Hybridoma Hbf-719, Producing Monoclonal Antibody Specific For Mouse Ly49f
Organization: University of California, Berkeley (UC Berkeley)
Hybridoma Yli-90, Producing Mabs Specific For Mouse Ly49i
Organization: University of California, Berkeley (UC Berkeley)
Hybridoma 20d5, Producing Mabs Specific For Mouse Nkg2a/c/e
Organization: University of California, Berkeley (UC Berkeley)
Organization: University of California, Berkeley (UC Berkeley)
Hybridoma Producing Monoclonal Antibodies To Murine Icos
Organization: University of California, Berkeley (UC Berkeley)
Cell Lines For Fusion Partners For T Cell Hybridomas
Organization: University of California, Berkeley (UC Berkeley)
Murine H60 Gene (plasmid) For Minor Histocompatibility Antigen
Organization: University of California, Berkeley (UC Berkeley)
Mouse Monoclonal Antibodies To Mouse Ctla-4
Organization: University of California, Berkeley (UC Berkeley)
Organization: University of California, Berkeley (UC Berkeley)
Hybridoma 16a11, Producing Monoclonal Antibody Specific For Mouse Nkg2a
Organization: University of California, Berkeley (UC Berkeley)
Monoclonal Antibodies Against Non-muscle Myosin Ii
Organization: University of California, Berkeley (UC Berkeley)
Organization: University of California, Berkeley (UC Berkeley)
Organization: University of California, Berkeley (UC Berkeley)
Integrated Microfluidic Cell Analysis System
Organization: University of California, Berkeley (UC Berkeley)
Microfluidic Sample Preparation And Impedimetric Detection Of Small Molecules
Organization: University of California, Berkeley (UC Berkeley)
Remote Optical Nano Switch For Localized Control Of Gene Interference
Organization: University of California, Berkeley (UC Berkeley)
Monoclonal Antibodies Specific For The Avermectin Antibodies
Organization: University of California, Berkeley (UC Berkeley)
Monoclonal Antibodies Specific For Triazine Herbicides Including Atrazine.
Organization: University of California, Berkeley (UC Berkeley)
Monoclonal Antibodies For Mouse Cd3 Chain
Organization: University of California, Berkeley (UC Berkeley)
Monoclonal Antibodies For Mouse Vg3 Chain
Organization: University of California, Berkeley (UC Berkeley)
Organization: University of California, Berkeley (UC Berkeley)
Recombinant Streptavidin-protein Chimeras Useful For Conjugation Of Molecules In The Immune System
Organization: University of California, Berkeley (UC Berkeley)
Rapid Assay And Detection Of Carbohydrates In Samples Using Molecular Markers
Organization: University of California, Berkeley (UC Berkeley)
Monoclonal Antibody For Isolation Of Oxidative Damaged Dna And Rna
Organization: University of California, Berkeley (UC Berkeley)
Organization: University of California, Berkeley (UC Berkeley)
Monoclonal Antibodies Specific For Cyclodiene Insecticides And Related Organochlorine Compounds
Organization: University of California, Berkeley (UC Berkeley)
Anti-SynDig1 Monoclonal Antibody
Organization: University of California, Davis (UC Davis)
Researchers at the University of California, Davis have developed anti-SynDig1 protein monoclonal antibody L42/17. The hybridoma is available for bailment as tangible research property. Antibodies are available for sale from the UC Davis/NIH Neuromab Facility. Additional information, including data sheets, can be found by searching the Neuromab Catalog at www.neuromab.org.
Immunoassays For Thiocarbamate Herbicides
Organization: University of California, Berkeley (UC Berkeley)
Monoclonal Antibodies To Apolipoprotein B Epitopes Of Human Low Density Lipoproteins
Organization: University of California, Berkeley (UC Berkeley)
Monoclonal Antibodies That Recognize Phenanthrene And Related Compounds
Organization: University of California, Berkeley (UC Berkeley)
Monoclonal Antibodies Against Murine Cd28 Antigen
Organization: University of California, Berkeley (UC Berkeley)
Monoclonal Antibodies For Detection Of Diuron And Related Phenylurea Herbicides
Organization: University of California, Berkeley (UC Berkeley)
Recombinant Antibodies For The Phenylurea Herbicide Diuron
Organization: University of California, Berkeley (UC Berkeley)
Monoclonal Antibodies Against Transcription Factor Spi And Cdna Clones For Tfiie
Organization: University of California, Berkeley (UC Berkeley)
Hybridomas Producing Monoclonal Antibodies To Basal Transcription Factors
Organization: University of California, Berkeley (UC Berkeley)
Monoclonal Antibodies Specific For The Major Virulence Antigen (v Antigen) Of Yersinia Pestis
Organization: University of California, Berkeley (UC Berkeley)
Organization: University of California, Berkeley (UC Berkeley)
Monoclonal Antibodies And Immunoassay Specific For The Toxic Congeners Of Polychlorinated Biphenyls
Organization: University of California, Berkeley (UC Berkeley)
Monoclonal Antibodies Against Ezrin
Organization: University of California, Berkeley (UC Berkeley)
Monoclonal Antibodies To Human Ku Autoantigen P85 And P70
Organization: University of California, Berkeley (UC Berkeley)
Species-specific Monoclonal Antibody To Chlamydia
Organization: University of California, Berkeley (UC Berkeley)
Monoclonal Antibody To Human Dna Polymerase Epsilon Catalytic Subunit
Organization: University of California, Berkeley (UC Berkeley)
Hamster Monoclonal Antibody Reactive With Notch1
Organization: University of California, Berkeley (UC Berkeley)
Hybrid IgA/IgG Polymeric Antibodies
Organization: University of California, Los Angeles (UCLA)
UCLA researchers have engineered IgA/IgG hybrid antibodies that combine characteristics of both IgA and IgG into a single immunoglobulin molecule. The IgG constant region was enhanced with the addition of IgA constant regions. This addition resulted in a molecule that has the pH stability of IgA and greater complement fixing activity than IgG due to its polymeric structure. By utilizing this technology, any variable region can be paired with the IgA/IgG hybrid to yield an immunoglobulin molecule with unique effector functions.
Production of Secretory IgA with Increased Stability
Organization: University of California, Los Angeles (UCLA)
UCLA researchers have developed a process to produce stable sIgA from a single mammalian cell in quantities practical for therapeutic uses. Vectors and cell lines for producing non-secretory IgA are readily available. In order to easily produce sIgA from these same cell types, a vector encoding the secretory signal is transfected into the IgA producing cell lines and stable transductants are cloned. In vitro and in vivo stability of the sIgA was measured and sIgA was found to have significantly greater stability than monoclonal IgA lacking ...
G2A GPCR Deficient Mouse Model and G2A Monoclonal Antibody
Organization: University of California, Los Angeles (UCLA)
Researchers at UCLA identified, characterized and patented the G2A GPCR. To help in the elucidation of the biological functions of G2A, these researchers have also developed a G2A GPCR deficient mouse model along with polyclonal and monoclonal antibodies useful for immunoprecipitations and Western blots. These tools may be used to study G2A to better understand its function in a variety of disorders and to also develop therapeutics that target this GPCR.
System to Produce Biotinylated Proteins
Organization: University of California, Los Angeles (UCLA)
UCLA researchers have developed a system that allows metabolic biotinylation of secreted proteins in vivo by both versions of biotin ligase (secreted and ER-retained). The system is a general approach for production of site-specific biotinylated proteins for streptavidin/avidin-biotin technology. The biotinylation technology also shortens and streamlines the lengthy process of traditional methods and reduces the chance of protein degradation during in vitro biotinylation by exogenous ligase. Not only is this system a significant ...
Novel Forms of Secreted Human IgE
Organization: University of California, Los Angeles (UCLA)
The invention is the discovery of several new forms of secreted IgE. These forms have potential applications in the diagnosis of immediate hypersensitivity-allergic disease. Previously thought to include only one or two forms, UCLA researchers have now determined that circulating IgE is expressed in four different forms. These four specific forms, which primarily differ at their C-terminal ends, are believed to have differential specificities and binding affinities for the cells that cause allergic reactions (mast cells/basophils). ...
Monoclonal Antibody Recognizing Human Abl2
Organization: University of California, Los Angeles (UCLA)
Researchers at UCLA have developed a monoclonal antibody that recognizes the human ABL2 (Abelson murine leukemia viral oncogene homolog 2) protein. It has been successfully used in standard immunoblot and immuno-precipitation techniques.
Organization: University of California, San Diego (UC San Diego)
This novel technology is a way to diagnose and treat CLL, breast cancer, and other cancers. A monoclonal antibody has been generated by UC San Diego against a receptor which is implicated in the progression of cancer. This protein named ROR1 is expressed on CLL B cells, but it is not present in normal lymphocytes.
Clinical Immunoassays to Determine Concentration of Monoclonal Antibodies
Organization: University of California, San Diego (UC San Diego)
This technology is a general method to develop assays to detect the circulating concentration of monoclonal antibodies in patients that have received them as treatment for cancer or other diseases. The method is applicable to any monoclonal antibody or other recombinant protein biologic therapy. In addition, two specific assays, one for rituximab and one for alemtuzumab, have been developed. Synthetic biotinylated peptides have been produced based on the sequence derived from phage displayed peptide libraries. UCSD researchers have then ...
Prediction of Cancer Treatment Side Effects Using a Patient's Protein Expression Profile
Organization: University of California, Los Angeles (UCLA)
Researchers at UCLA have discovered that by analyzing a readily available biological sample from a patient a clinician can determine whether or not the patient is at risk of developing severe normal tissue side effects, such as mucositis, from cancer treatment. This invention uses a patient's protein expression profile to identify at risk patients prior to treatment. With this information, clinicians will be able to modify treatment plans to prevent these side effects and/or enable the clinician to direct expensive supportive care ...
Hybridoma Secreting Antibody To Mouse Cd-1
Organization: University of California, Los Angeles (UCLA)
The innovation allows researchers to study and analyze CD1d expression and function. The monoclonal antibody is compatible with various laboratory techniques, including immunoprecipitation, flow cytometry, and immunohistochemistry.
Anti-Human Deoxycytidine Kinase (dCK) Monoclonal Antibody
Organization: University of California, Los Angeles (UCLA)
A Novel Glycopolymer to Enhance Protein Stability
Organization: University of California, Los Angeles (UCLA)
Dr. Heather Maynard and colleagues at UCLA have developed a novel glycopolymer to enhance the stabilization of proteins in response to various environmental stressors, including lyophilization and heat. The glycopolymer incorporates the disaccharide trehalose, which is frequently used as a preservative in foods and is Generally Regarded As Safe. The trehalose-based polymer developed by the Maynard group is prepared in such a way to have end-groups that the polymer can, if desired, be directly attached to the biomolecule. Direct ...
Antibody-based Agents for Imaging in vivo CD8 Expression
Organization: University of California, Los Angeles (UCLA)
Researchers in the laboratory of Dr. Anna Wu in the department of Molecular & Medical Pharmacology have developed an antibody-based agent for imaging in vivo CD8 expression in mouse models of disease. The present invention utilizes the sequencing of the variable domains of anti-mouse-CD8 from hybridomas for the subsequent engineering into antibody fragments, such as, but not limited to, scFv's, diabodies, minibodies and scFv-Fc's. The antibody is subsequently conjugated to a radioisotope or fluorophore to enable visualization of ...
Non-Invasive, Sensitive Diagnostic for Viral Myocarditis
Organization: University of California, San Diego (UC San Diego)
Researchers from UC San Diego have identified altered forms of a protein complex present in cardiac muscle (the dystrophin-glyoprotein complex), which is associated with dilated cardiomyopathy caused by enteroviruses. Specifically, the presence of a unique, enteroviral protease 2A-cleavage product provides a highly specific and sensitive biomarker for enteroviral-mediated cleavage of dystrophin.
Stat5-specific protease polypeptide
Organization: Columbia Tech Ventures
Antibodies having modified carbohydrate content and methods of preparation and use
Organization: Columbia Tech Ventures
Organization: STC UNM
A collaboration of researchers at the University of New Mexico, University of Nevada, Reno, and University of Minnesota have developed a system of both vaccine formulation and method of immunization to better diagnose, treat, and even prevent infection of B. anthracis through utilizing the glutamic acid capsule that surrounds the bacteria. The capsules that surround this specific bacteria, made of yDPGA or yLPGA, prevent phagocytosis of the pathogen and thus allow for its replication within the subject’s blood and tissues. The vaccine ...
Monoclonal antibody enhancing T cell reactivity
Organization: Columbia Tech Ventures
Surface plasmon resonance biosensor for measurement of anti-glycolipid antibody levels in neuropathy
Organization: Columbia Tech Ventures
Monoclonal Antibody Recognizing Tau Phosphorylated at Tyrosine 18
Organization: University of Iowa Res Foundation
A Protective Vaccine and Monoclonal Antibody for Francisella tularensis
Organization: University of Iowa Res Foundation
Depleting the C5/C5a Component of Complement to Enhance the Efficacy of Vaccines
Organization: University of Iowa Res Foundation
Ultrasound System and methods for quantifying analytes and point of care diagnostics
Organization: McGill University
Method for Screening for Agents Inhibiting Chloride Intracellular Channels
Organization: Columbia Tech Ventures
Novel therapeutic treatment of chronic obstructive pulmonary disease
Organization: Columbia Tech Ventures
A mouse model of cutaneous inflammation and hyperpigmentation
Organization: Columbia Tech Ventures
Stat5-specific protease polypeptide
Organization: Columbia Tech Ventures
Detection of Cancer Cells Using the Prostate Tumor Inducing Gene
Organization: Columbia Tech Ventures
In-office or home test kit for periodontal disease based on analysis of enzyme activity in saliva
Organization: Columbia Tech Ventures
Immunoassay using antibodies directed to the reaction product of enzymes used as labels
Organization: Columbia Tech Ventures
Organization: Columbia Tech Ventures
Domain-modified constant region antibodies
Organization: Columbia Tech Ventures
Antibodies having modified carbohydrate content and methods of preparation and use
Organization: Columbia Tech Ventures
Chimeric antibodies with receptor binding ligands in place of their constant region
Organization: Columbia Tech Ventures
MONOCLONAL ANTIBODY TO THE PROTEIN NCOA6
Organization: National Institutes of Health/National Human Genome Research Institute
Organization: STC UNM
A novel method for overcoming the disadvantages associated with existing fluorescent antibodies and for generating stable recombinant antibodies has been developed and is exemplified by a novel multi-colored fluorescent antibody platform in the design, assembly, intracellular bacterial production and purification of a panel of novel antibody fluorescent protein fusion constructs. The panel of multi-colored antibodies was assembled by genetically incorporating fluorescent proteins as bridging scaffolds (rather than by chemical linking ...
Polyclonal Antibodies Raised Against Full Length Human Syndecan-1
Organization: STC UNM
A method for developing a polyclonal antibodies raised against full-length human Syndecan-1.
Monoclonal Antibodies Directed Against Human Blood Coagulation Proteins
Organization: STC UNM
A number of murine monoclonal antibodies are available directed at human proteins involved in blood coagulation: anti- human Factor VII (and VIIa), anti -human Factor X (and Factor Xa), anti-human Prothrombin, anti human Protein C (and activated Protein C) and anti- human Factor IX (and Factor IXa). All of these are conformationally-specific and calcium dependent and have been used in affinity chromatography to isolate the protein of interest from conditioned media in a single step. Also available are two calcium-independent ...
Monoclonal Antibody for In Vitro Assessment of Therapeutic Agents Based on Factor IX
Organization: STC UNM
Monoclonal Antibody for In Vitro Assessment of Therapeutic Agents Based on Factor IX. These antibodies are available for licensing on a non-exclusive basis for research purposes – either individually or as a group. Licenses in the field of diagnostics or other commercial purposes are also available.
Monoclonal Antibodies for In Vitro Assessment of Therapeutic Agents Based on Factor VIIa
Organization: STC UNM
Monoclonal Antibodies for In Vitro Assessment of Therapeutic Agents Based on Factor VIIa. These antibodies are available for licensing on a non-exclusive basis for research purposes – either individually or as a group. Licenses in the field of diagnostics or other commercial purposes are also available.
Prostate carcinoma analysis antibody (Nkx3.1 (mouse) antibody)
Organization: Columbia Tech Ventures
Prostate carcinoma analysis antibody (Nkx3.1 (human) antibody)
Organization: Columbia Tech Ventures
Antibody for quantifying carcinogen exposure in humans (BPDE-DNA (5D11) monoclonal antibody)
Organization: Columbia Tech Ventures
Antibody for monitoring BDPE exposure in human (BDPE-I monoclonal antibody (8E11))
Organization: Columbia Tech Ventures
Antibody for detecting transcription factors involved in interferon signaling (anti-mouse STAT2)
Organization: Columbia Tech Ventures
Antibody for detecting transcription factors involved in interferon signaling (anti-human STAT2)
Organization: Columbia Tech Ventures
Antibody for detecting transcription factors involved in interferon signaling (anti-human STAT1)
Organization: Columbia Tech Ventures
Novel Method for Increasing Antibody Production
Organization: University Health Network
Monoclonal antibodies are effectively used in a variety of therapeutic, diagnostic, and research applications. In fact, recombinant proteins, including monoclonal antibodies, contribute to a significant portion of the $60 billion worldwide therapeutic protein market. Similarly, the market for diagnostic proteins is on the order of $40 billion worldwide and a large component of this market is monoclonal antibodies. As well, there is a large growing market for monoclonal antibodies for research purposes. It is estimated that majority of the ...
A Specific Antibody for Mechanistic Indicators of Plaque Formation in Neurodegenerative Disease
Organization: WORLDiscoveries - University of Western Ontario
A leading Amyotrophic Lateral Sclerosis (ALS) scientist at Robarts has generated an antibody that binds an epitope that holds promise to be sensitive and specific to ALS. The polyclonal antibody is fully developed, is very clean, and is specific to an epitope modification on a well accepted ALS disease related protein. This offering represents the only commercially available antibody for this specific epitope. A scientific publication relating to the epitope is pending in the near future.
Organization: University of Michigan
Research Tool: Monoclonal antibody UMCD6
Hybridoma Cell Line 1E7E8, Monoclonal Antibody UMCD2
Organization: University of Michigan
Research Tool: Monoclonal antibody 1E7E8 specific for the CD2 molecule
Monoclonal Antibody UMCD6 (Hybridoma 3F1B5), Specific for the T Cell Glycoprotein CD6
Organization: University of Michigan
Research Tool: Monoclonal antibody UMCD6 (hybridoma 3F7B5), specific for the T cell glycoprotein CD6
Monoclonal Antibody UM7F8, Specific for the Human Cell Surface Antigen CD98 (4F2)
Organization: University of Michigan
Research Tool: Monoclonal antibody UM7F8 specific for the human cell surface antigen CD98 (4F2)
1B6 Hybridoma Secreting a Murine Monoclonal Antibody Specific for Rat CD11b
Organization: University of Michigan
Research Tool: Murine monoclonal antibody specific for the rat form of the beta2 integrin glycoprotein alpha subunit CD11b
Hybridoma Cells Producing Monoclonal Antibodies to Human Podocalyxin (Antibodies 3D3, 4F10, and 2A4)
Organization: University of Michigan
Hybridoma Cells Producing Monoclonal Antibodies to Human Podocalyxin (Antibodies 3D3, 4F10, and 2A4)
Monoclonal Antibody Specific for the von Willebrand Factor
Organization: University of Michigan
Monoclonal Antibody Specific for the von Willebrand Factor
A Novel Dendritic Cell Associated Antigen
Organization: University of Pittsburgh
Hybridoma Cell Line 74-5H7 Producing Monoclonal Antibodies Against L1 Cell Adhesion Molecule
Organization: University of Pittsburgh
Organization: University of Pittsburgh
ANK-1 monoclonal antibody and hybridoma cells producing this antibody
Organization: University of Pittsburgh
Preparation and isolation of a potent immune serum that targets the yeast Hsp110 chaperone, Sse1p
Organization: University of Pittsburgh
Monoclonal Antibodies for the diagnosis and treatment of basal breast carcinoma
Organization: University of Pittsburgh
anti-HLA-DR, -DQ and -DP beta chains mAb LGII-612.14 and hybridoma cell line
Organization: University of Pittsburgh
Anti-beta 2-microglobulin (beta 2-mu) MoAb NAMB-1 and hybridoma cell line
Organization: University of Pittsburgh
Anti-HLA-A2,A28 MAb KS1 and hybridoma cell line
Organization: University of Pittsburgh
Anti-HLA-A2,B17 MAb HO-2 and hybridoma cell line
Organization: University of Pittsburgh
anti-alpha(v) mAb VF33-ST100 and hybridoma cell line
Organization: University of Pittsburgh
anti-alpha(v) mAb TP36.1 and hybridoma cell line
Organization: University of Pittsburgh
anti-alpha(v) beta 3 mAb VF27.263.15 and hybridoma cell line
Organization: University of Pittsburgh
anti-alpha(v) beta 3 mAb VF27-312 and hybridoma cell line
Organization: University of Pittsburgh
anti-ICAM-1 mAb CL203.4 and hybridoma cell line
Organization: University of Pittsburgh
anti-HLA-A mAbs LGIII-147.4.1 and hybridoma cell line
Organization: University of Pittsburgh
anti-ICAM-1 mAb VF27-516 and hybridoma cell line
Organization: University of Pittsburgh
Monoclonal antibody to exon 2 peptides of Merkel cell polyomavirus
Organization: University of Pittsburgh
Anti-human and mouse PTEN-induced kinase-1 (PINK1) polyclonal antibodies
Organization: University of Pittsburgh
Monoclonal antibody (Clone J3) to human basement membrane collagen type IV
Organization: University of Pittsburgh
Organization: University of Pittsburgh
Monoclonal anti-migfilin (also known as FBLP1, FBLP-1) antibody
Organization: University of Pittsburgh
Organization: University of Pittsburgh
Gamma-parvin (also known as PARVG) monoclonal antibody.
Organization: University of Pittsburgh
Alpha-actinin 4 (ACTN4) monoclonal antibody.
Organization: University of Pittsburgh
Beta-parvin (also known as Affixin) monoclonal antibodies
Organization: University of Pittsburgh
Organization: University of Pittsburgh
Mouse Monoclonal Antibody Specific for Merkel Cell Polyomavirus-Viral Capsid Protein
Organization: University of Pittsburgh
Mouse Monoclonal Antibody Specific for Merkel Cell Polyomavirus- Small T antigen
Organization: University of Pittsburgh
PLUNC as a detection and therapeutic agent to protect against microbial infection
Organization: University of Pittsburgh
Natural Feed Additive Combats Gastrointestinal Infection in Livestock and Poultry
Organization: Wisconsin Alumni Research Foundation (WARF)
Reaping billion dollar losses every year, coccidiosis is a common protozoan infection of agricultural animals that targets the digestive system. Its symptoms include weight loss, diarrhea and suppressed growth. Drugs have been developed to treat the disease, but are being phased out in markets in Europe and Asia due to potential hazards to both animals and humans. The emergence of resistant strains also has spurred interest in fresh tactics. Vaccines are one alternative, but they take several weeks to become effective and may undermine ...
Eight Hybridoma Cell Lines Producing Monoclonal Antibodies Against La Crosse Encephalitis Virus
Organization: Wisconsin Alumni Research Foundation (WARF)
La Crosse virus is an etiologic agent for childhood encephalitis in North America. Structurally, the nucleocapsid of this virus is enclosed by a host-derived lipid envelope holding two virion glycoproteins, G1 and G2. These proteins are believed to be responsible for attachment of the virus to cells. Although several researchers had been successful at producing specific monoclonal antibodies against the larger G1, production of a monoclonal antibody directed against the G2 viral protein has not been reported. UW-Madison researchers have now ...
Organization: Wisconsin Alumni Research Foundation (WARF)
The gene HERG1 encodes a potassium-selective ion channel important in maintaining normal cardiac rhythm and cell growth. UW-Madison researchers have developed polyclonal IgG antibodies against 15-amino acid peptide sequences unique to HERG1. The peptide sequences were selected based on their antigenicity, surface probability and conservation among species. These sera have been validated for use in Western blot, immuno-precipitation and histological characterization of native and expressed HERG channels. In validation tests, they strongly ...
Novel Assay for Measuring Stress-Induced CREB Phosphorylation
Organization: Wisconsin Alumni Research Foundation (WARF)
CREB is a transcription factor that has been implicated as a critical regulator of neuronal function and survival. Knockout mice lacking CREB exhibit a disease similar to ataxia telangiectasia, a neurodegenerative disease primarily seen in children that is characterized by neurodegeneration, extreme sensitivity to UV and ionizing radiation and high susceptibility to cancers. UW-Madison researchers have developed a phospho-specific antibody that can be used to monitor stress-induced phosphorylation of the CREB transcription factor. CREB is ...
Method and Device for Separating Particles By Size
Organization: Wisconsin Alumni Research Foundation (WARF)
The need for integrated protein bioassays has led to a proliferation of assays involving antibodies immobilized on beads. A variety of techniques have been developed to separate particles by size; however, these methods have a number of limitations, including cost, power or voltage requirements, sample volume requirements, difficulty of manufacture, complex construction, moving parts, measurement quality, reliability and response time. UW-Madison researchers have developed a method and device for separating mixed particles of different sizes ...
Monoclonal Antibody to Eukaryotic RNA Polymerase II
Organization: Wisconsin Alumni Research Foundation (WARF)
The enzyme RNA polymerase II synthesizes precursors to mRNAs and small nuclear RNAs. Sequence analysis of the largest subunit of RNA polymerase II has revealed an unusual heptapeptide repeat at the molecule’s C-terminal end. This domain is highly conserved in mammals and yeast, and less well conserved in Drosophila. UW-Madison researchers have developed a monoclonal antibody against the largest subunit of RNA polymerase II. The antibody was isolated by using RNA polymerase II purified from wheat germ. It reacts with the largest, ...
A Polyclonal Antibody Directed Against the C-Terminus of HERG
Organization: Wisconsin Alumni Research Foundation (WARF)
HERG (human ether-a-go-go-related gene) encodes a potassium ion channel important for maintaining normal cardiac rhythm and cell growth. UW-Madison researchers have developed a versatile polyclonal anti-HERG antibody. This antibody was developed in 1997 using a fusion protein that included 181 amino acids from the C-terminus of HERG. It detects HERG 1a and 1b. The antibody’s specificity has been tested by Western blot, immunohistochemical and immunoprecipitation assays, including histological characterization of native and expressed ...
Monoclonal Antibodies Specific for the Cytoplasmic Gamma-actin Isoform
Organization: Wisconsin Alumni Research Foundation (WARF)
The six mammalian actin genes encode a highly conserved family of cytoskeletal protein isoforms important in cell structure, mechanical stability, and motility. Actin isoforms exhibit tissue-specific expression and distinct subcellular distributions, suggesting that each isoform may serve a different function. Although commercially available monoclonal antibodies exist for most isoforms of actin, the only available antibodies specific for the gamma-cytoplasmic isoform were extremely variable polyclonal antibodies. UW-Madison researchers have ...
Increasing Production of Egg Antibodies
Organization: Wisconsin Alumni Research Foundation (WARF)
Powdered egg containing antibodies has many commercial applications. It is used in immunological assays, and may be incorporated into animal feed as an alternative to antibiotics that are used to enhance weight gain. UW-Madison researchers have developed a method of increasing the production of antibodies in an egg. Enriched eggs currently are formed by feeding or injecting chickens with antigens and various other components. The inventors discovered that adding different molecular components of a compound present in all Gram-positive ...
Blood-Brain Barrier Targeting Antibodies
Organization: Wisconsin Alumni Research Foundation (WARF)
Even though therapeutic compounds have been developed for neurologic disorders, such as Alzheimer’s disease, these conditions remain difficult to treat, largely because of the blood-brain barrier. The blood-brain barrier interferes with drug delivery, allowing only those molecules that are lipophilic and have low molecular weight (less than 500 Da) to enter the brain from the bloodstream. More than 98 percent of small molecule pharmaceuticals and nearly 100 percent of protein and gene therapeutics cannot pass through this barrier. ...
Mouse Monoclonal Antibodies to the Human Nuclear Receptor, Estrogen-Related Receptor Alpha
Organization: Wisconsin Alumni Research Foundation (WARF)
Estrogen-related receptor alpha (ERRalpha) is a recently discovered biomarker and treatment target for breast cancer (See WARF reference number P03031US). However, few, if any reliable monoclonal antibodies to ERRalpha are commercially available. UW-Madison researchers have developed high quality, well characterized monoclonal antibodies to ERRalpha. Because some of these antibodies bind antigen tightly but release it when eluted with a mixture of salt and a polyol, they are ideal for use in immunoaffinity chromatography.
Antibodies to NGAL for Detection of Cancer and Kidney Damage
Organization: Wisconsin Alumni Research Foundation (WARF)
Neutrophil gelatinase-associated lipocalin (NGAL) is a recently discovered cytokine. Because many types of cells produce NGAL in response to injury, it provides a biomarker for several chronic conditions. NGAL can be used in a diagnostic assay to detect certain types of cancer cells (see WARF reference number P95225US). It serves as a strong indicator of kidney damage, and may be useful to monitor other chronic conditions as well. UW-Madison researchers have developed purified antibodies to NGAL. These unique antibodies can be used in ...
A Monoclonal Antibody Specific for Brain Alpha-Dystroglycan
Organization: Wisconsin Alumni Research Foundation (WARF)
The transmembrane protein dystroglycan is associated with autosomal recessive muscular dystrophies. Although dystroglycan is glycosylated in a tissue-specific manner, no commercial monoclonal antibodies capable of differentiating between the various tissue-specific glycoforms of alpha-dystroglycan currently exist. UW-Madison researchers have developed mAb 2238E2D2, an IgG2b isotype monoclonal antibody that specifically recognizes brain alpha-dystroglycan from a variety of species. They developed this monoclonal antibody by immunizing mice ...
Antibody That Recognizes the Sulfonylurea Receptor 1 Isoform
Organization: Wisconsin Alumni Research Foundation (WARF)
Sulfonylurea receptors (SURs) regulate the opening and closing of ATP-sensitive potassium channels (KATP). They play important roles in insulin secretion in the pancreas and myocardial protection in the heart. At least two isoforms of SUR exist, and the mRNA from each isoform can be spliced in different ways to result in further variants of SUR. The SUR1 isoform encodes the high-affinity sulfonylurea receptor, which is found in pancreatic beta-cells, while the SUR2 isoform encodes the low-affinity sulfonylurea receptor, which is mainly ...
Mouse Monoclonal Antibodies to Epstein-Barr Virus Nuclear Antigen 1 (EBNA1)
Organization: Wisconsin Alumni Research Foundation (WARF)
Epstein-Barr virus (EBV) is one of the most common human viruses and also is associated with some rare cancers. EBV nuclear antigen 1 (EBNA-1) is involved in replication and transcriptional regulation of EBV. EBNA-1 plays an important role in the malignancies associated with EBV infection, including Burkitt’s lymphoma, some Hodgkin’s disease and post-transplant lymphoproliferative diseases. Monoclonal antibodies (mAbs) would be a good tool to characterize EBNA-1; however, few high quality mAbs to EBNA-1 currently ...
Monoclonal Antibodies Against Cardiovirus Proteins
Organization: Wisconsin Alumni Research Foundation (WARF)
Encephalomyocarditis virus, a cardiovirus, causes encephalitis and myocarditis. Rodents are natural hosts for this virus and can transmit it to other animals. Encephalomyocarditis virus is a widespread problem in swine, causing sudden death and reproductive failure, and potentially leading to severe economic losses for pork producers. UW-Madison researchers have developed monoclonal and polyclonal antibodies against recombinant cardiovirus proteins. The antibodies can be used for specific molecular recognition of the cardiovirus proteins in ...
Mouse Monoclonal Antibody Against the Sigma70 Subunit of <i>E. coli</i> RNA Polymerase
Organization: Wisconsin Alumni Research Foundation (WARF)
RNA polymerase is the enzyme responsible for DNA transcription, the process of making an RNA copy of a DNA gene sequence. Transcription begins at DNA sequences called promoters. In bacteria, a sigma factor directs the polymerase to gene promoters, ensuring that transcription occurs only where it is supposed to. UW-Madison researchers have developed a mouse monoclonal antibody (mAb) against an E. coli sigma factor. Known as 2F8, this mAb specifically recognizes the sigma70 transcription factor, which is the main sigma factor.
Mouse Monoclonal Antibody Against the Alpha Subunit of <i>E. coli</i> RNA Polymerase
Organization: Wisconsin Alumni Research Foundation (WARF)
RNA polymerase is the enzyme responsible for DNA transcription, the process of making an RNA copy of a DNA gene sequence. The core RNA polymerase enzyme in E. coli consists of two alpha subunits, a beta subunit and a beta` subunit. The alpha subunits are responsible for assembling the subunits into the enzyme. UW-Madison researchers have developed a mouse monoclonal antibody, known as 4RA1, which specifically recognizes the alpha subunit of E. coli RNA polymerase.
Polyclonal Antibodies Against Cardiovirus 2A, 2B, 2C, 3C, 3A, 3B and 3AB Proteins
Organization: Wisconsin Alumni Research Foundation (WARF)
Encephalomyocarditis virus, a cardiovirus, causes encephalitis and myocarditis. It is a widespread problem in swine, causing sudden death and reproductive failure and potentially leading to severe economic losses for pork producers. This virus also infects exotic mammals such as elephants and llamas. UW-Madison researchers have developed polyclonal mouse antibodies against recombinant cardiovirus proteins. The antibodies can be used for specific molecular recognition of the cardiovirus proteins in vitro or in vivo using experimental ...
Polyclonal Antibodies Against Equine Rhinitis A Virus 3AB, 3C and 3D Proteins
Organization: Wisconsin Alumni Research Foundation (WARF)
Equine rhinitis A virus, formerly known as equine rhinovirus 1, causes a respiratory disease in horses similar to the common cold in humans. No vaccines against this virus are available commercially and no specific antiviral treatment exists. UW-Madison researchers have developed polyclonal antibodies against recombinant proteins from equine rhinitis A virus. The antibodies can be used for specific molecular recognition of the equine rhinitis A virus proteins in vitro or in vivo using experimental techniques such as ELISA (enzyme linked ...
Antibody That Recognizes the Sulfonylurea Receptor 2 Isoform
Organization: Wisconsin Alumni Research Foundation (WARF)
Sulfonylurea receptors (SURs) regulate the opening and closing of ATP-sensitive potassium channels (KATP). They play important roles in insulin secretion in the pancreas and myocardial protection in the heart. At least two isoforms of SUR exist, and the mRNA from each isoform can be spliced in different ways to result in further variants of SUR. The SUR1 isoform encodes the high-affinity sulfonylurea receptor, which is found in pancreatic beta-cells, while the SUR2 isoform encodes the low-affinity sulfonylurea receptor, which is mainly ...
Antibody That Recognizes Both Isoforms of the Sulfonylurea Receptor
Organization: Wisconsin Alumni Research Foundation (WARF)
Sulfonylurea receptors (SURs) regulate the opening and closing of ATP-sensitive potassium channels (KATP). They play important roles in insulin secretion in the pancreas and myocardial protection in the heart. At least two isoforms of SUR exist, and the mRNA from each isoform can be spliced in different ways to result in further variants of SUR. The SUR1 isoform encodes the high-affinity sulfonylurea receptor, which is found in pancreatic beta-cells, while the SUR2 isoform encodes the low-affinity sulfonylurea receptor, which is mainly ...
Antibody That Recognizes the 2A Variant of the Sulfonylurea Receptor
Organization: Wisconsin Alumni Research Foundation (WARF)
Sulfonylurea receptors (SURs) regulate the opening and closing of ATP-sensitive potassium channels (KATP). They play important roles in insulin secretion in the pancreas and myocardial protection in the heart. At least two isoforms of SUR exist, and the mRNA from each isoform can be spliced in different ways to result in further variants of SUR. The SUR1 isoform encodes the high-affinity sulfonylurea receptor, which is found in pancreatic beta-cells, while the SUR2 isoform encodes the low-affinity sulfonylurea receptor, which is mainly ...
Antibody That Recognizes the 2B Variant of the Sulfonylurea Receptor
Organization: Wisconsin Alumni Research Foundation (WARF)
Sulfonylurea receptors (SURs) regulate the opening and closing of ATP-sensitive potassium channels (KATP). They play important roles in insulin secretion in the pancreas and myocardial protection in the heart. At least two isoforms of SUR exist, and the mRNA from each isoform can be spliced in different ways to result in further variants of SUR. The SUR1 isoform encodes the high-affinity sulfonylurea receptor, which is found in pancreatic beta-cells, while the SUR2 isoform encodes the low-affinity sulfonylurea receptor, which is mainly ...
Peptide Mimotypes Bind Natural Antibodies, Modulate Autoimmunity
Organization: Wisconsin Alumni Research Foundation (WARF)
Naturally occurring antibodies are important components of the innate immune system. They participate in clearing pathogens, enhancing immune responses and preventing autoimmunity. The molecular recognition mechanisms underlying the functions of natural antibodies are poorly understood, largely because most monoclonal natural antibodies are polyspecific and recognize a variety of antigens that share no obvious structural similarity. This polyspecificity is likely an inherent property of natural antibodies that enables a ...
Monoclonal Antibodies Against Human Rhinovirus Type 16, a Model Virus for the Common Cold
Organization: Wisconsin Alumni Research Foundation (WARF)
Human rhinoviruses are the most frequent cause of the common cold. Human rhinovirus 16 (HRV16) is a widely used model virus for studying the common cold, virus-induced asthma and rhinovirus transmission, as well as in evaluating anti-rhinovirus drugs in humans. A UW-Madison researcher has developed a mouse monoclonal antibody (mAb) against HRV16. This mAb, called mAb16-7, detects HRV16 and HRV1A by specifically recognizing the viral capsid protein VP2. It can be used in a variety of experimental techniques, such as immuno-staining and ...
Polyclonal Antibodies to CARM1
Organization: Wisconsin Alumni Research Foundation (WARF)
The enzyme CARM1 (coactivator associated arginine methyltransferase1) plays an important role in controlling development and cell growth. This nuclear hormone receptor co-activator is involved in the activation of several transcription factors, including NF-kB, p53, E2F1 and steroid receptors, such as the estrogen receptors. Standard analysis techniques, such as immunohistochemistry, can be used to improve our understanding of the function of CARM1 in vivo; however, high quality antibodies are needed for these analyses. A UW-Madison ...
Monoclonal Antibodies That Recognize Recombinant Mengovirus 3C Proteinase
Organization: Wisconsin Alumni Research Foundation (WARF)
Mengovirus is a picornavirus that causes encephalomyocarditis in humans and other animals. UW-Madison researchers have created hybridoma cell lines producing monoclonal antibodies raised to recombinant Mengo 3C protease; these antibodies are designated 6D10, 8F10 and 10C6. This material may be used in the specific molecular recognition and detection of protein 3C (protease), which is encoded within the genome of Mengovirus of the family Picornaviradae.
Monoclonal and Polyclonal Antibodies Raised to Recombinant Mengovirus 3D Polymerase
Organization: Wisconsin Alumni Research Foundation (WARF)
Mengovirus is a picornavirus that causes encephalomyocarditis in humans and other animals. UW-Madison researchers have created polyclonal antibodies and various hybridoma cell lines producing monoclonal antibodies raised to recombinant Mengo 3D polymerase. These antibodies are designated 1B6, 3C5, 10D3, 2F11, 3C6, 2F6, 3B7, 10B3, 1D6, 5F6, and 8D10. This material can be used in the specific molecular recognition and detection of the protein 3D polymerase that is encoded within the genome of Mengovirus in the family Picornavirae.
Using Corticosteroids to Increase Egg Antibody Titer
Organization: Wisconsin Alumni Research Foundation (WARF)
Eggs are used to produce antibodies for use as an alternative to antibiotics in animal feed supplements, as diagnostic reagents in clinical and research laboratory settings or as active and passive vaccines. Antibody production generally involves immunizing an egg-laying animal with an antigen. Then antibodies to the antigen accumulate in the eggs, particularly the egg yolks. UW-Madison researchers have developed a method of increasing egg antibody titer by more than two-fold. Increasing antibody titer significantly reduces ...
GFAbs – GFP-Based Biosensors with the Binding Properties of Antibodies
Organization: Wisconsin Alumni Research Foundation (WARF)
Antibodies are used widely in biological and medical research to bind to and help isolate molecules of interest. However, the reduced stability and production yields of antibody fragments frequently utilized in in vitro platforms has led to attempts to create alternative binding scaffolds. By mutagenizing solvent-exposed loop regions or inserting diverse loop repertoires into non-antibody protein scaffolds, specific binding attributes can be conferred to proteins with desirable properties, such as high stability and production ...
Phospho-Specific Antibody to Study Circadian Rhythm Gene PER2
Organization: Wisconsin Alumni Research Foundation (WARF)
Circadian rhythms refer to the 24-hour cycles of biological processes that promote daily functioning. The synchronization of an organism’s internal clock to external cues like light and darkness is called entrainment. In mammals, entrainment is critically influenced by the gene known as PERIOD2 (or PER2). Mutation of this gene can lead to lifelong sleep abnormalities. Specifically, mutations can result in differently phosphorylated proteins. Previous studies have not been able to directly identify PER2 proteins phosphorylated at specific ...
Organization: Wisconsin Alumni Research Foundation (WARF)
Aova Technologies Inc. is a Wisconsin agricultural biotechnology company commercializing patented antibody products produced in chicken eggs. Product use, in simple and complex diets, has shown improved feed efficiency and enhanced growth rate of animals regardless of antibiotic usage. By implementing the company's products, animal producers and feed ingredient manufacturers gain natural products that improve their bottom line.
Organization: Wisconsin Alumni Research Foundation (WARF)
Metabiologics focuses on the development and manufacturing of novel botulinum toxin products used for general research applications. Along with the toxin products, Metabiologics also provides customers with antibodies and bioassay services.
Organization: Wisconsin Alumni Research Foundation (WARF)
NeoClone Biotechnology International LLC excels in the development of monoclonal antibodies. Using its expertise in the development process, NeoClone can produce high affinity antibodies in significantly less time than the rest of the industry. NeoClone currently is servicing the reagent and diagnostic markets (400+ customers in more than 30 countries) and is enhancing its proprietary process to provide services to the therapeutic market.
