Details |
Project Title | THERMOSTABLE RNA-GUIDED ENDONUCLEASES AND METHODS OF USE THEREOF (GeoCas9) | |
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Track Code | 27624 | |
Website | https://techtransfer.universityofcalifornia.edu/NCD/27624.html?utm_source=AUTMGTP&utm_medium=webpage&utm_term=ncdid_27624&utm_campaign=TechWebsites | |
Short Description | None | |
Abstract | The CRISPR-Cas system is now understood to confer bacteria and archaea with acquired immunity against phage and viruses. CRISPR-Cas systems consist of Cas proteins, which are involved in acquisition, targeting and cleavage of foreign DNA or RNA, and a CRISPR array, which includes direct repeats flanking short spacer sequences that guide Cas proteins to their targets. The programmable nature of these systems has facilitated their use as a versatile technology that is revolutionizing the field of genome manipulation. There is a need in the art for additional CRISPR-Cas systems with improved cleavage and manipulation under a variety of conditions and ones that are particularly thermostable under those conditions.
UC researchers discovered a new type of RNA-guided endonuclease (GeoCas9) and variants of GeoCas9. GeoCas9 was found to be stable and enzymatically active in a temperature range of from 15°C to 75°C and has extended lifetime in human plasma. With evidence that GeoCas9 maintains cleavage activity at mesophilic temperatures, the ability of GeoCas9 to edit mammalian genomes was then assessed. The researchers found that when comparing the editing efficiency for both GeoCas9 and SpyCas9, similar editing efficiencies by both proteins were observed, demonstrating that GeoCas9 is an effective alternative to SpyCas9 for genome editing in mammalian cells. Similar to CRISPR-Cas9, GeoCas9 enzymes are expected to have a wide variety of applications in genome editing and nucleic acid manipulation.
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Tags | CRISPR, gene editing, genome, gene therapy, cell biology, Genomics, biological, Research Tools, Therapeutics, Nucleic Acids/DNA/RNA | |
Posted Date | Aug 15, 2017 11:28 AM |
Advantages |
Functions under different conditions than current CRISPR-Cas9 proteins (e.g, thermostable and enzymatically active in a wide temperature range)
Has an extended lifetime in human plasma
Shown to work in mammalian cells
Potential Applications |
Genome editing
Genetic engineering
Gene therapy
Research tools (e.g., high-throughput screening of gene functions in cell lines and in vivo)
Creation of transgenic animal models
Additional Information |
Publication
A thermostable Cas9 with increased lifetime in human plasma
Additional Technologies by these Inventors
- COMPOSITIONS AND METHODS FOR IDENTIFYING HOST CELL TARGET PROTEINS FOR TREATING RNA VIRUS INFECTIONS
- Genome Editing via LNP-Based Delivery of Efficient and Stable CRISPR-Cas Editors
- Type III CRISPR-Cas System for Robust RNA Knockdown and Imaging in Eukaryotes
- Cas12-mediated DNA Detection Reporter Molecules
- Improved guide RNA and Protein Design for CasX-based Gene Editing Platform
- Cas13a/C2c2 - A Dual Function Programmable RNA Endoribonuclease
- RNA-directed Cleavage and Modification of DNA using CasY (CRISPR-CasY)
- CasX Nickase Designs, Tans Cleavage Designs & Structure
- In Vivo Gene Editing Of Tau Locus Via Liponanoparticle Delivery
- A Dual-RNA Guided CasZ Gene Editing Technology
- CRISPR-CAS EFFECTOR POLYPEPTIDES AND METHODS OF USE THEREOF (“Cas-VariPhi”)
- Modifications To Cas9 For Passive-Delivery Into Cells
- A Protein Inhibitor Of Cas9
- RNA-directed Cleavage and Modification of DNA using CasX (CRISPR-CasX)
- Compositions and Methods for Genome Editing
- Split-Cas9 For Regulatable Genome Engineering
- NANOPORE MEMBRANE DEVICE AND METHODS OF USE THEREOF
- Methods to Interfere with Prokaryotic and Phage Translation and Noncoding RNA
- CRISPR CASY COMPOSITIONS AND METHODS OF USE
- Single Conjugative Vector for Genome Editing by RNA-guided Transposition
- Improved Cas12a Proteins for Accurate and Efficient Genome Editing
- CRISPR-CAS EFFECTOR POLYPEPTIDES AND METHODS OF USE THEREOF
- Engineered/Variant Hyperactive CRISPR CasPhi Enzymes And Methods Of Use Thereof
- Engineering Cas12a Genome Editors with Minimized Trans-Activity
- Methods Of Use Of Cas12L/CasLambda In Plants
- Type V CRISPR/CAS Effector Proteins for Cleaving ssDNA and Detecting Target DNA
- Structure-Guided Methods Of Cas9-Mediated Genome Engineering
- Endoribonucleases For Rna Detection And Analysis
- Efficient Site-Specific Integration Of New Genetic Information Into Human Cells
- CRISPR-Cas Effector Polypeptides and Methods of Use Thereof
- Class 2 CRISPR/Cas COMPOSITIONS AND METHODS OF USE
- Compositions and Methods of Use for Variant Csy4 Endoribonucleases
- Identification Of Sites For Internal Insertions Into Cas9
- Methods and Compositions for Controlling Gene Expression by RNA Processing
Tech ID/UC Case
27624/2017-151-0
Related Cases
2017-151-0
Contact Information |
Name : Terri Sale
Title :
Department :
Email : terri.sale@berkeley.edu
Phone : 510-643-4219
Address :
Principal Investigator |
Name : Jennifer Doudna
Department :
Name : Lucas Harrington
Department :
Name : Nikos Kyrpides
Department :
Name : David Paez-Espino
Department :
Intellectual Property |
Patent Number : 11692184
Patent Title :
Patent Application Date :
Patent Publication Date :
Patent Issue Date : Jul 4, 2023
Patent Link : http://www.google.com/patents/US11692184