Directly Contact Organization
Save Project as XML
Details |
| Project Title | An Efficient Gene Editing Approach to Create Transgene-free Mutant Plants | |
|---|---|---|
| Track Code | 28790 | |
| Website | https://techtransfer.universityofcalifornia.edu/NCD/28790.html?utm_source=AUTMGTP&utm_medium=webpage&utm_term=ncdid_28790&utm_campaign=TechWebsites | |
| Short Description | Researchers at UC San Diego have developed an effective strategy to reliably isolate Cas9-free T2 plants that contain stably heritable mutations in Arabidopsis. The technology uses a cassette that enables the expression of a visual marker (e.g., luminescent or fluorescent marker) gene under the control of a strong promoter inserted into the CRISPR/Cas9 vector. The visual marker cassette allows one to visually select Cas9-free plants at T2 generation. | |
| Abstract | Effective isolation of targeted mutations generated by CRISPR/Cas9 requires not only reasonable editing efficiency, but also an easy method to screen for the mutations. Editing events generated by CRISPR/Cas9 are normally identified by restriction enzyme digestion of PCR fragments or by in vitro digestion using purified Cas9 protein. Both methods are time-consuming and laborious. Simplified screening methods are urgently needed. | |
| Tags | Cas9-mediated genome editing, CRISPR, Mutagenesis, Arabidopsis, screening of mutations, fluorescent marker, Transgenics, Genomics | |
| Posted Date | Jul 21, 2017 7:49 AM | |
Advantages |
The current technology is much faster and less laborious compared to traditional PCR based methods. The method is suitable for use across a broad range of plant species.
Potential Applications |
Crop improvement through genome editing. This invention allows a very fast and efficient identification of Cas9-free plants with the desired genome modifications. This is especially important for crop improvement using CRISPR/cas9 genome editing technology because of public concern over transgenes.
Additional Information |
State Of Development
Arabidopsis was used as a model to generate targeted mutations and demonstrate that the technology could successfully identify that the mutations were stably transmitted.
Intellectual Property Info
A provisional patent has been submitted and the technology is available for licensing.
Related Materials
Tech ID/UC Case
28790/2016-309-0
Related Cases
2016-309-0
Contact Information |
Name : University of California, San Diego Office of Innovation and Commercialization
Title :
Department :
Email : licensing@ucsd.edu
Phone : 858.534.5815
Address :
Principal Investigator |
Intellectual Property |
Patent Number :
Patent Title :
Patent Application Date :
Patent Publication Date :
Patent Issue Date : Sep 13, 2018
Patent Link : http://www.google.com/patents/WO2018165249
Patent Number : 00486461
Patent Title :
Patent Application Date :
Patent Publication Date :
Patent Issue Date : Feb 13, 2020
Patent Link : http://www.google.com/patents/US00486461